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Program Schedule of Indo-US Workshop
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Day 1: Monday 21st March, 2022
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8:00-9:00
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Registrationoftheparticipants,Breakfast
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9:00-10:00
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Inauguration, Welcome,Introduction, Overview of theworkshop
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Dr.B.Suresh
Dr.SurinderSingh
Dr.Manjunatha
Dr.H.Basavanagowdappa
Dr. Purnima Madhivanan
Dr.LeeRiley
Dr.MatamVijaykumar
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10:00-10:50
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Principles of MolecularEpidemiology
Part 1: Introduction to MolecularEpidemiology; definitions;distinguishing molecularepidemiologyfromtaxonomy/
phylogeny
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Dr. Lee RileyChairpersons
Dr.M.N.Sumana
Dr.N.B.Ramachandra
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10:50-11:00
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Break
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11:00-12:00
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Principles of MolecularEpidemiology
Part 2: Overview of molecularmicrobiologymethodsusedin
epidemiology
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Dr. Lee RileyChairpersons
Dr.B.Madhu
Dr.N.B.Ramachandra
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12:00-13:00
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Lunch
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13:00-14:00
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Basic Epidemiological Concepts
Part 1: Study design Overview
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Dr.PurnimaMadhivanan
Chairperson
Dr. Praveen KulkarniDr.KiranmayeeMuralidhar
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14:00-15:10
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BasicEpidemiologicalConcepts
Part2:Epidemiological methods 1
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Dr.PurnimaMadhivanan
Chairperson
Dr. Praveen KulkarniDr.KiranmayeeMuralidhar
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15:10-15:30
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Break
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15:30-17:00
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Part 1: Scientific manuscript writingandGrant writing
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Dr.KarlKrupp
Chairpersons
Dr.PrashantVishwanathDr.T.Rajeshkumar
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17:00-17:30
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Conclusion of the day and high tea
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Day 2: Tuesday 22nd March 2022
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8:00-9:00
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Practices of MolecularEpidemiology
Part1:Salmonellosisanddrug-resistanturinarytractinfections.
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Dr. Lee RileyChairpersons
Dr.M.N.Sumana
Dr.ManjunathShetty
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9:00-10:00
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Practices of MolecularEpidemiology
Part 2: Overview of next-generationsequencing techniques: WGS
platforms,DNAlibrarypreparation
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Dr. Kerry CooperChairpersons
Dr.AkilaPrashant
Dr.RamachandraKini
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10:00-10:15
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Break
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10:15-11:15
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BasicEpidemiologicalConcepts
Part3:Epidemiological methods 2
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Dr. Purnima Madhivanan
Chairpersons
Dr. D. Sunil Kumar Dr. B. Prashantha
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11:15-12:15
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Practices of MolecularEpidemiology
Part3:Overviewofbioinformatics
workflowtoanalyzeWGSdata
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Dr. Kerry CooperChairpersons
Dr.AkilaPrashant
Dr.SutturMalini
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12:15-13:15
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Lunch
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13:15-15:30
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Basicepidemiologicconcepts
Part4:Surveillance
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Dr.PurnimaMadhivanan
Chairpersons
Dr.SudhirNayakDr.AnilBilimale
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15:30-15:45
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Break
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15:45-17:00
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Part 2: Scientific manuscript writingandGrant writing
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Dr.KarlKrupp
Chairpersons
Dr.DivyaPrasannakumarDr.AshaSrinivasan
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Day 3: Wednesday 23rd March 2022
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8:10-9:00
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Basic epidemiologic concepts
Part 5: Outbreak investigations
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Dr. Purnima Madhivanan
Chairpersons
Dr. Raghavendra Dr. C. Amrutha Valli
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09:00-9:50
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Practices of MolecularEpidemiology
Part4:Real-worldapplicationofwholegenomesequencingfor
molecularepidemiology
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Dr. Kerry Cooper Chairpersons Dr. Akila Prashant
Dr. C. Amrutha Valli
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10:00-10:15
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Break
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10:15-11:15
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Modularexercise
Multidrug-resistant Klebsiellapneumoniaeinfectioninahospital
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Coordinators
Dr.RashmiP.MahaleDr.PrakashAlami
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11:15-12:15
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Practices of MolecularEpidemiology
Part5:Hostinnateimmunityandgutmicrobiotainhealthanddisease
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Dr.MatamVijay-Kumar
Chairpersons
Dr.PrasannaKumarDr.P.V.Ravindra
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12:15-13:15
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Lunch
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13:15-14:15
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Practices of MolecularEpidemiology
Part6:Hostinnateimmunityand
gutmicrobiotainhealthanddisease
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Dr.MatamVijay-Kumar
Chairpersons
Dr.PrasannaKumarDr.P.V.Ravindra
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14:15-14:30
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Break
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14:30-16:30
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Student abstract presentationsand discussion and feedback frominstructors
Coordinators
Dr.N.K.SwethaandDr.D.Devanand
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05 studentswill beselected for presentationbased on their submittedabstractquality (10minutespresentation&10minutes
discussionperstudent)
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Day 04: Thursday 24th March, 2022
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8:30-9:00
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BasicsofPCRbyDr.SubbaRaoV.Madhunapantula
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9:00-11:00
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Evaluation of the performance of the candidates anddiscussion–Dr.KerryCooper
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11:00-11:15
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Break
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11::15-1:30
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Isolation,QualityassessmentandQuantificationofDNA,PreparationofAgarose Gel andElectrophoresis
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13:30-14:00
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Lunch
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14:00-15:00
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Settingupthe MLSTPCR reaction
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15:00-15:30
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Basicsofprimerdesigning–Dr.AkilaPrashant
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15:30-15:45
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Break
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15:45-16:00
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AnalysisofthePCRproductsbyagarosegelelectrophoresis
–Samplepreparationandloading
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16:00-17:00
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Discussiononthedaysactivityandplanfornextdayscheduleand observation of the electrophoressed PCR products inthegeldocumentationunit
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Day 05: Friday 25th March, 2022
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8:30-9:30
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MultiplexPCR:Protocoldevelopmentandapplications–Dr.D.Devanand
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9:30-10:30
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SetupamultiplexPCRreaction–DemonstrationbyMr.AnshuK.Yadav
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10:30-10:45
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Break
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10:45-11:30
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BasicsofRT-PCR–Dr.RajeshThirumalappa
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11:30-12:30
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ApplicationsofRT-PCRindiseasediagnosis–Dr.Prasanna Kumar
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12:30-13:00
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Agarosegelelectrophoresisofthesamples
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13:00-13:30
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SetupanRT-PCRreaction
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13:30-14:00
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Lunch
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14:00-14:30
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Analysisoftheelectrophoresedsamples
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14:30-16:00
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VisittothemoleculargeneticslaboratoryintheJSSHospital
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16:00-16:30
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AnalysisofRT-PCRdata
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16:30-17:00
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Discussion
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17:00-17:30
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Valedictoryfunction(AddressbyDr.M.N.SumaandDr.PrashantVishwanath)
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Demonstration of advanced microscopy and automated Western Blotting
Day 06: Saturday 26th March 2022
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9:30-10:45
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Lifetime based species separation using ConfocalMicroscope–Dr.ManojManna(ProductandApplicationManager,LeicaMicrosystems)
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10:45-11:00
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Break
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11:00-13:30
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Demonstrationandhandsonexperienceofconfocal microscopy (Batch 1)
Sample preparation and demonstration of JESS-Automated Western Blotting (Batch 2)
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13:30-14:00
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Demonstrationandhandsonexperienceofconfocal microscopy (Batch 2)
Sample preparation and demonstration of JESS-Automated Western Blotting (Batch 1)
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LoadingproteinsamplesintotheJESS(Batch1&2)
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Day 07: Sunday 27th March 2022
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9:30-10:45
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JESS-AutomatedWesternBlottinganditsapplications–HiteshSharma(FieldApplicationScientist,Bio-Techne)
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10:45-11:00
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Break
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11:00-13:00
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DiscussionanddataanalysisofdatageneratedfromJESS
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13:00-13:30
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Lunch
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13:30-14:00
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Valedictoryfunction(AddressbyDr.M.N.SumaandDr.PrashanthVishwanath)
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